- B.S., Microbiology, University of Puerto Rico/Arecibo (2015)
- Toxin A and toxin B from Clostridium difficile and their effects on a three-dimensional blood-brain barrier model
- Ph.D., Neuroethology, University of Puerto Rico-Rio Piedras (2015)
- B.S., Coastal Marine Biology, University of Puerto Rico-Humacao (2005)
- Axo-somatic mixed synapses on supramedullary and spinal cord dorsal neurons
Irma I. Torres-Vazquez
- M.S., Biology, University of Puerto Rico-Rio Piedras (1994)
- B.S., Biology, University of Puerto Rico-Mayaguez (1984)
- Axo-somatic mixed synapses between spinal inhibitory interneurons (i.e., VIa) and spinal motor neurons
Prem Singh Thapa-Chetri
- Ph.D., Photonics, Oklahoma State University (2008)
- M.S., Electrical Engineering, Oklahoma State University (2002)
- B.S., Electrical and Electronics Engineering, Bangladesh Institute of Technology (1995)
- Nanomaterials and their effects on a three-dimensional blood-brain barrier model
Post-Baccalaureate Research Education Program (PREP) Fellow
Neuroscience/MAI Laboratory Research Director
Eduardo Rosa-Molinar's is a senior scientist and director of the Microscopy and Analytical Imaging Resources Core Laboratory (MAI). His appointment is equally divided between that of director of MAI and professor in the Department of Pharmacology and Toxicology.
Rosa-Molinar brings to the MAI experience in successfully administering research projects (i.e., hiring and managing staff, managing regulatory issues [animal and human use and experimentation; laboratory and chemical safety], developing balanced and fiscally responsible budgets), collaborating with other researchers, developing research grant proposals, and in writing and peer-reviewing publications and extended abstracts. As a result of prior administrative experience, he is aware of the importance of frequent communication among project members and of constructing a realistic development plan, timeline, and budget.
As a faculty member, his research goal is to decipher a “gap-junction-coupled motor pattern-generating neural microcircuit” that consists of a small number of gap-junction-coupled neurons forming unique patterns, underlies an innate behavior, is usually inflexible and is triggered by a stimulus.
Serving as principal investigator (PI), co-PI, participant, or collaborator of university and National Science Foundation (NSF) and National Institutes of Health (NIH)-funded competitive grant awards assisted him in laying and continuing to build the foundation required to achieve his long-term research and MAI bio-technology resource goal by:
- developing new functional nano-materials that span spatial domains from sub-nanometer to microns
- enhancing existing and developing new methods to use those nano-materials to selectively label neural cells and the protein network that constitutes the cytomatrix at the pre-synaptic and post -synaptic active zone of “mixed” synapses, a juxtaposition of chemical and electrical (i.e., gap junction) synaptic components associated with the identifiable neurons
- improving existing and developing new tissue contrast reagents and techniques
- developing imaging methods for high-resolution three-dimensional (3-D) photon-based and high-throughput 3-D electron-based microscopies to collect and disseminate 3-D teravoxel or petavoxel image data
- testing computer algorithms that can reveal complex patterns and relationships
- using as a “reference species” the adult Western Mosquitofish Gambusia affinis (Mosquitofish), a species that has unique advantages and is ideally suited for “mapping” a gap-junction-coupled motor pattern-generating neural microcircuit and for testing and refining novel tools and methods. I develop quantitative immuno-correlative photon- and electron-based imaging technologies required to image and analyze in 3-D the nano-scale membrane organization of mixed synapses’ pre-and post-synaptic membrane proteins.
The above-described approaches have resulted in productive research projects in an area of high relevance: finding wiring diagrams that underlie simple behaviors and seeing how the wiring diagrams differ among individuals. Deciphering a “gap-junction-coupled motor pattern-generating neural microcircuit” that underlies an innate behavior will assist in understanding what accounts for individual and sex differences in innate behavior and how, over time, such as in aging, wiring diagrams and innate behavior change. In summary, his expertise and experience have prepared him to design and lead the research projects underway in his laboratory at the University of Kansas.
Eduardo Rosa-Molinar and his group research focuses on neurotechnology, specifically developing/refining reagents, tools, and workflows for multi-scale multi-modal correlated volume resin microscopies, in order to determine the three-dimensional nano-scale geometry and chemical composition of synapses. We are particularly interested in “mixed synapses”, a poorly studied synapse that combines the features of both chemical and electrical (e,g., gap junction) synapses.
Laboratory rotation in the Rosa-Molinar laboratory provides broad-based training and experience with microscopy tools and workflows needed in cellular, synaptic, and circuit neuroscience.
- Postdoctoral Training, Creighton University School of Medicine, Omaha, NE (1999)
- Ph.D., Medical Sciences: Anatomy, Cell Biology, and Neuroscience, University of Nebraska Medical Center. Omaha, NE (1997)
- B.S., Natural Sciences, The University of Alabama, Tuscaloosa, AL (1994)
Eduardo Rosa-Molinar's research focuses on the development and application of tools and workflows for multi-scale multi-modal correlated volume microscopies and three-dimensional volume reconstruction to unravel synaptic geometry.